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A3279
October 13, 2014
1:00 PM - 3:00 PM
Room Hall B1-Area C
mRNA Expression Profiles in Response to Diazoxide Postconditioning Hypoxia Reoxygenation Injury in Rat Myocardial Cell
Wen-ting Sun, M.Med., Song Cao, M.Med., Li Zhao, M.Med., Xingkui Liu, M.Med., Tian Yu, M.Med.
Zunyi Medical College, Zunyi City, China
mRNA expression profiles in response to Diazoxide Postconditioning hypoxia reoxygenation injury in rat myocardial cell

Objectives

To study the effect of Diazoxide postconditioning on the gene expression of myocardial cell hypoxia reoxygenation injury model and investigate the involved molecular mechanism.

Methods

Animal study was conducted with reference to the care and use guideline of laboratory animals (Home Office, United Kingdom). Cardiomyocytes are isolated from male adult Sprague-Dawley rat (250-300)g left ventricle by Langendorff-perfusion and collagenase-II digestion. Adult SD rat myocardial cell hypoxia reoxygenation injury model was established.The Sprague-Dawley the Diazoxide postconditioning group (group DZ). male rat myocardial cell are randomly divided into 2 groups. i.e. the control group (group C), Group C hypoxia 45 min,then reoxygenation 60 min; group DZ hypoxia 45 min, then 5 mins with diazoxide before reoxygenation , then continue reoxygenation 55 min.. The totle RNA was Extracted from the two groups for Illumina sequencing.Their potential ontology and pathway were analyzed by using the DAVID Bioinformatics Resources.

Results

By analysis we found 363 genes being up-regulated and 340 genes being down-regulated(Q-Value﹥0.8) in the myocardial cell after Diazoxide postconditioning. Some significant different genes are listed in Table 1 and Table 2. Analyzed by GO software we found these genes were involved in cellular process, metabolic process, cell part, membrane part, binding,catalytic activity,and so on(Pvalue﹤0.05). This part result are shown in Fig. 1. Analyzed by KEGG pathway,we found these genes were involved in 75 signaling pathways(Pvalue﹤0.05), including metabolic pathways, citrate cycle (TCA cycle),glycerophospholipid, inositol phosphate metabolic, Pyruvate metabolism, cell cycle,petose phosphate pathway,VEGF signaling pathway,and so on. This part result are shown in Table 3 and Fig. 2.

Conclusion

The results show that intervention of Diazoxide may regulate some significant genes expression,such as MT-ND6, Sdha, Cycs, Ogdh, most of which are associated with the mitochondrial respiratory chain and the energy metabolism.

Key Words mitoKATP; DGE; Diazoxide; Myocardium ischemia-reperfusion injury (MIRI); Pharmacological postconditioning
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