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October 24, 2016
10/24/2016 1:00:00 PM - 10/24/2016 3:00:00 PM
Room Hall F Foyer-Area D
Detection of Infarct Zone Using a 3T MRI Clinical Scanner in Stroked Rats: A Comparative Study
Yulia Grinshpun, M.D., Benjamin F. Gruenbaum, M.D., Ruslan Kuts, M.D., Shaun E. Gruenbaum, M.D., Vladislav Zvenigorodsky, M.D., Matthew Boyko, Ph.D.
Soroka University Medical Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel
Disclosures:  Y. Grinshpun: None. B.F. Gruenbaum: None. R. Kuts: None. S.E. Gruenbaum: None. V. Zvenigorodsky: None. M. Boyko: None.
Introduction: Stroke is associated with a very high morbidity and mortality; therefore investigations of its mechanisms and pathophysiology are critical for the development of new drugs and therapies. The majority of previous animal models of stroke, which aimed at identifying the extent and location of brain injury following stroke, required animal euthanasia. In addition to ethical considerations, euthanasia also negated the possibility for follow-up studies in these rats. In this study we developed a new protocol for the evaluation of stroke lesions in a 3T MRI clinical scanner (Ingenia, Philips Medical Systems, Best, The Netherlands) using an eight-channel receive-only coil. We validated a new MRI protocol by comparing infarcted zone via MRI with the “gold standard” of histological examination.

Materials and Methods: Ten male Wistar rats were randomly assigned into one of 2 groups. The first group was subjected to middle cerebral artery occlusion (MCAO) (stroke group) and second group was used as a control (sham) group. 1 week after surgery the rats were examined in the clinical MRI 3T scanner for evaluation of infarct zone. Immediately after completion of the MRI procedure the rats were sacrificed and their brains were taken for histological examination. MRI results were compared to measurements obtained by histological examination of the same rats.

Results: Percent of infarct zone was measured using 3 different techniques (sequences): T2, DWI and ADC. Assessment of brain lesion calculated by MRI techniques: stroke group T2=21.4%±3.3%, DWI=12.3%±4.1% and ADC=4.3±2.5%. Histological examination revealed an infarcted zone of 19.1%±5.1%. The correlation between histological examination and T2 was R=0.91 (P<0.01); ADC was R=0.45 (P<0.05) and DWI R=0.69 (P<0.01).

Conclusions: The results suggest that a clinical 3T MRI may be successfully applied for the measurement of infarcted zone volume at 1 week after ischemic stroke in rats, which strongly correlated with the same measures achieved by histological examination. T2 most accurately reflected the infarct zone in a rat model of stroke to continue for 1 week. In-vivo evaluation of the infarct zone using MRI techniques gives us an opportunity to investigate the dynamics of stoke condition, to try new therapies and to measure efficacy of treatment.
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